Corn Cobs Hemicelluloses Isolation Method Comparison and its Characterization with Infra Red Spectrophotometry (FTIR) and High Performance Liquid Chromatography (HPLC)

Hemicellulose is a component of Non-Starch Polysaccarides (NSP) contained in agricultural byproduct and can be separated from the other components. Various studies about hemicelluloses isolation procedures from various agricultural byproduct materials have been conducted including corn cobs. Objective of this research is to modify the isolation method and compare with other procedures of isolation method and characterization of corn cobs hemicellulose. To get the optimum of hemicelluloses yield, the isolating corn cobs hemicellulose using three procedures that had been done by other researchers and the modified procedure that had been conducted by the researcher himself. Characterization of corn cobs hemicellulose was done by infra red spectrophotometry (FTIR), high performance liquid chromatography (HPLC) with column Shim pack VP ODS (4.6 x 250 mm) using a UV Vis detector at a wavelength of 280 nm with an isocratic elution system in aquabides solvent and a flow rate of 0.8 mL/min. The modified procedure provided the greatest yield of corn cobs hemicellulose which is 12.04% with characteristic FTIR region of 1820-1600 cm and vibration widened near 3800-3000 cm. This means that the corn cobs hemicellulose having functional groups within the structure, that is the carbonyl group, hydroxyl and carboxylic groups. The results showed that the yield of corn cobs hemicellulose with characteristic HPLC chromatograms from all procedures of isolation had a retention time of 1.8 minutes. In conclusion that the four isolation methods had the same structure and the same characteristic with FTIR dan HPLC.The modified method showed the highest yield compared to other methods and produced environmentally friendly waste.